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Inside the test for Indicative Qualities colony morphology and sign reaction really should be comparable to that attained With all the Earlier permitted batch of media.
It can be supplemented with added parts or indicators to support unique growth needs or to detect certain metabolic pursuits or reactions.
In GPT failure circumstances, all over again prepare fresh new lot of dehydrated media through the exact media container and accomplish GPT all over again.
Regularly observe the cultures for growth, noting any observations or abnormalities. This can assist you identify Should the growth promotion test is profitable or if any concerns need to be tackled.
Verify the Actual physical affliction of dehydrated media powder, if lumps are formed from the media discard that media.
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Just after the desired incubation period of time, notice the plates for growth and count the colonies, if any, obtained in Each and every plate and history the results in Annexure-II.
To complete Growth promotion test on new media ton, consider Anybody media container within the new large amount and perform test with microorganisms as proven in Desk-I.
Stable Medium: Average the volume of colonies from your new batch of medium and the quantity of colonies from the previously approved batch of medium. For The brand new batch of medium for being authorized, the subsequent acceptance criteria must be satisfied for each microorganism tested: The typical range of colonies on the new batch in the medium should be “similar” to the average variety of colonies on the Formerly permitted batch. A quantitative definition of “comparable” is just not established through the click here USP.
In addition to new great deal media, any approved media ton shall be tested with all test parameters for comparison research.
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dimaksudkan untuk kontrol positif apakah media benar-benar dapat ditumbuhi bakteri sesuai jumlah bakteri yang ditanam pada media tersebut, apabila dari media yang ditanam bakteri yang jumlahnya misalkan seventy five cfu dan ternyata jumlah bakteri yang tumbuh kurang dari fifty% dari jumlah bakteri yang ditanam dapat disimpulkan bahwa pengujian jumlah bakteri dan jamur read more pada sampel produk tidak valid karena media tidak dapat menumbuhkan bakteri sebagaimana mestinya.